protein

PNKP Protein, Human (His-SUMO)

HY-P71620

20 μg;50 μg;100 μg

170;315;504 USD

PNKP Protein, Human (His-SUMO)

proteins

E. coli

20 μg;50 μg;100 μg

170;315;504 USD

PNKP protein plays a key role in DNA damage repair through NHEJ and BER pathways. Its dual catalytic activity includes removal of the 3'-phosphate group from the DNA backbone and phosphorylation of the 5'-hydroxyl group, ensuring compatibility of DNA ends during extension and ligation. PNKP Protein, Human (His-SUMO) is the recombinant human-derived PNKP protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Human

PNKP Protein assumes a crucial role in the repair of DNA damage, actively participating in both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Employing its two catalytic activities, PNKP plays a pivotal role in maintaining DNA termini compatibility for subsequent extension and ligation processes. It accomplishes this by either removing 3'-phosphates from the DNA backbone or phosphorylating 5'-hydroxyl groups on the ribose sugar. This dual functionality highlights PNKP's significance in resolving various types of DNA lesions, ensuring the integrity of the genome and contributing to the intricate network of cellular mechanisms dedicated to DNA repair and maintenance.

2''(3'')-polynucleotidase; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; Polynucleotide kinase-3''-phosphatase

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Room temperature in continental US; may vary elsewhere.