protein

Frataxin/FXN Protein, Cynomolgus (His)

HY-P71593

5 μg;10 μg;50 μg

130;220;620 USD

Frataxin/FXN Protein, Cynomolgus (His)

proteins

E. coli

5 μg;10 μg;50 μg

130;220;620 USD

Frataxin (FXN) protein activates persulfide transfer, which is critical for [2Fe-2S] cluster assembly. It accelerates sulfur transfer from NFS1 persulfide to ISCU and small thiols, resulting in oversulfide and sulfide release. Frataxin/FXN Protein, Cynomolgus (His) is the recombinant cynomolgus-derived Frataxin/FXN protein, expressed by E. coli , with N-6*His labeled tag. The total length of Frataxin/FXN Protein, Cynomolgus (His) is 130 a.a., with molecular weight (affected by relative charge) of ~22 KDa.

Cynomolgus

Frataxin (FXN) Protein serves as an activator in the persulfide transfer process within the core iron-sulfur cluster (ISC) assembly complex, crucial for [2Fe-2S] cluster assembly. It facilitates the acceleration of sulfur transfer from NFS1 persulfide intermediate to ISCU and small thiols like L-cysteine and glutathione, leading to persulfuration and sulfide release. During [2Fe-2S] cluster assembly, FXN binds ferrous ions and is released upon the addition of both L-cysteine and reduced FDX2. The ISC assembly complex, consisting of FXN, NFS1, LYRM4, NDUFAB1, and FDX2, initiates de novo synthesis of [2Fe-2S] clusters, transferring them to chaperone proteins such as HSCB, HSPA9, and GLRX5. FXN may play a role in protecting against iron-catalyzed oxidative stress, displaying ferroxidase activity in its oligomeric form. It could potentially store large amounts of iron in the form of a ferrihydrite mineral through oligomerization, although the physiological relevance remains uncertain. FXN may function as an iron chaperone protein, safeguarding the aconitase [4Fe-4S]2+ cluster from disassembly and promoting enzyme reactivation. Additionally, it might serve as a high-affinity iron-binding partner for FECH, contributing to the terminal step in mitochondrial heme biosynthesis. FXN modulates the RNA-binding activity of ACO1, potentially participates in cytoplasmic iron-sulfur protein biogenesis, and could contribute to oxidative stress resistance and overall cell survival.

FXN; FRDA1; QnpA-13971; Frataxin; mitochondrial; Fxn; EC 1.16.3.1) [Cleaved into: Frataxin intermediate form; Frataxin mature form]

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

Room temperature in continental US; may vary elsewhere.