protein

TetR Protein, E.coli (His)

HY-P71520A

5 μg;10 μg;50 μg;100 μg

62;100;280;450 USD

TetR Protein, E.coli (His)

proteins

E. coli

5 μg;10 μg;50 μg;100 μg

62;100;280;450 USD

TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His) is the recombinant E.coli-derived TetR, expressed by E. coli , with N-6*His labeled tag. ,

E.coli

The TetR protein operates as a repressor for the tetracycline resistance element, with its N-terminal region adopting a helix-turn-helix structure that facilitates DNA binding. Tetracycline binding to TetR induces a conformational change, diminishing the repressor's affinity for the operator sites of the tetracycline resistance gene (tetA) promoter. This dynamic interaction between TetR and tetracycline serves as a regulatory mechanism, modulating the expression of genes associated with tetracycline resistance. In the presence of tetracycline, TetR undergoes alterations in its binding properties, allowing for the derepression of the tetA promoter and enabling the expression of genes involved in resistance to tetracycline.

tetR; Tetracycline repressor protein class B from transposon Tn10

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 8.0, 8% trehalose, 8% mannitol and 0.02% Tween80.

Room temperature in continental US; may vary elsewhere.