protein

TetR Protein, E.coli (His-SUMO)

HY-P71520

20 μg;50 μg;100 μg

280;615;985 USD

TetR Protein, E.coli (His-SUMO)

proteins

E. coli

20 μg;50 μg;100 μg

280;615;985 USD

TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His-SUMO) is the recombinant E. coli-derived TetR protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

E.coli

The TetR protein operates as a repressor for the tetracycline resistance element, with its N-terminal region adopting a helix-turn-helix structure that facilitates DNA binding. Tetracycline binding to TetR induces a conformational change, diminishing the repressor's affinity for the operator sites of the tetracycline resistance gene (tetA) promoter. This dynamic interaction between TetR and tetracycline serves as a regulatory mechanism, modulating the expression of genes associated with tetracycline resistance. In the presence of tetracycline, TetR undergoes alterations in its binding properties, allowing for the derepression of the tetA promoter and enabling the expression of genes involved in resistance to tetracycline.

tetR; Tetracycline repressor protein class B from transposon Tn10

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

Room temperature in continental US; may vary elsewhere.