protein

GFP Protein, Aequorea victoria (His)

HY-P71461

5 μg;10 μg;50 μg;100 μg;500 μg;1 mg

30;48;125;200;600;960 USD

GFP Protein, Aequorea victoria (His)

proteins

E. coli

5 μg;10 μg;50 μg;100 μg;500 μg;1 mg

30;48;125;200;600;960 USD

The green fluorescent protein (GFP), Aequorea victoria is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, found in organisms including Aequorea victoria, corals, sea anemones, zoanithids, copepods and lancelets. GFP Protein has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm, while its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP Protein acts as an energy transfer receptor and actively transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer, which is used as expression of reporter genes. GFP Protein, Aequorea victoria (His) is a recombinant jellyfish Aequorea victoria GFP protein with N-6*His labeled tag that consists of 238 amino acids, which is expressed in E. coli [1] [2] [3] [4] [5] [6] [7] .

Others

GFP fluorescence occurs when aequorin interacts with Ca 2+ ions, inducing a blue glow in Aequorea victoria . Some of this luminescent energy is transferred to the GFP, shifting the overall color towards green, making GFP protein enabling the visualization of specific proteins and cellular structures in living organisms [3] . GFP Protein has a β barrel structure consisting of eleven β-strands with a pleated sheet arrangement, with an α helix containing the covalently bonded chromophore 4-(p-hydroxybenzylidene)imidazolidin-5-one (HBI) running through the center [4] . In a live cell imaging experiment, on the one hand, GFP Protein directly attaches to a protein of interest to indicate a successful transfection of a gene of interest. On the other hand, GFP Protein that containing a mutation where the fluorescence will change from green to yellow over time is used to study the state of protein production such as recently activated, continuously activated or recently deactivated [6] . GFP Protein is used widely in cancer research to label and track cancer cells. GFP Protein-labelled cancer cells have been used to model metastasis, the process by which cancer cells spread to distant organs [7] .

GFPGreen fluorescent protein

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4 or PBS, pH 7.4, 8% trehalose.

Room temperature in continental US; may vary elsewhere.