protein

UBE2I Protein, Human (GST)

HY-P71403

10 μg;50 μg

50;150 USD

UBE2I Protein, Human (GST)

proteins

E. coli

10 μg;50 μg

50;150 USD

The UBE2I protein is a key player in protein sumoylation, accepting the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. UBE2I cooperates with E3 ligases such as RANBP2, CBX4, and ZNF451 to catalyze the covalent attachment of these SUMO proteins to target proteins, including key substrates FOXL2 and KAT5. UBE2I Protein, Human (GST) is the recombinant human-derived UBE2I protein, expressed by E. coli , with N-GST labeled tag. The total length of UBE2I Protein, Human (GST) is 158 a.a., with molecular weight of ~40.0 kDa.

Human

UBE2I, a crucial player in the protein sumoylation process, demonstrates versatility by accepting ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4, and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. Collaborating with various E3 ligases, such as RANBP2, CBX4, and ZNF451, UBE2I catalyzes the covalent attachment of these SUMO proteins to target proteins. This enzymatic activity extends to the formation of poly-SUMO chains, emphasizing its role in diverse cellular processes. UBE2I is essential for sumoylation events involving critical substrates like FOXL2 and KAT5, contributing to nuclear architecture and chromosome segregation. Furthermore, UBE2I-mediated sumoylation plays a pivotal role in specific protein modifications, including sumoylation of p53/TP53 at 'Lys-386' and ERCC6, the latter being indispensable for its transcription-coupled nucleotide excision repair activity.

SUMO-Conjugating Enzyme UBC9; SUMO-Protein Ligase; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Conjugating Enzyme E2 I; Ubiquitin-Protein Ligase I; p18; UBE2I; UBC9; UBCE9

Greater than 95% as determined by reducing SDS-PAGE.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.

Shipping with dry ice.