protein

UBE2A Protein, Human (GST-His)

HY-P71397

5 μg;10 μg;50 μg;100 μg

35;55;130;200 USD

UBE2A Protein, Human (GST-His)

proteins

E. coli

5 μg;10 μg;50 μg;100 μg

35;55;130;200 USD

UBE2A cooperates with the E3 enzyme BRE1 to promote the attachment of ubiquitin to proteins, specifically histone H2B at "Lys-120", forming H2BK120ub1. This modification serves as a tag for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is critical for the formation of H3K4me and H3K79me. UBE2A Protein, Human (GST-His) is the recombinant human-derived UBE2A protein, expressed by E. coli , with N-6*His, N-GST labeled tag. The total length of UBE2A Protein, Human (GST-His) is 152 a.a., with molecular weight of ~52.0 kDa.

Human

UBE2A, as an integral component of the ubiquitin-proteasome system, serves as a conduit for ubiquitin transfer from the E1 complex to target proteins, catalyzing their covalent attachment. Collaborating with the E3 enzyme BRE1 (RNF20 and/or RNF40), UBE2A plays a pivotal role in transcriptional regulation by orchestrating the monoubiquitination of histone H2B at 'Lys-120,' resulting in the formation of H2BK120ub1. This modification serves as a distinctive mark for epigenetic transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is essential for the subsequent formation of H3K4me and H3K79me. In addition to its role in histone ubiquitination, UBE2A exhibits catalytic activity in vitro, mediating both 'Lys-11' and 'Lys-48'-linked polyubiquitination. Moreover, UBE2A is indispensable for the postreplication repair of UV-damaged DNA, highlighting its multifaceted involvement in fundamental cellular processes.

Ubiquitin-Conjugating Enzyme E2 A; RAD6 Homolog A; HR6A; hHR6A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; UBE2A; RAD6A

Greater than 80% as determined by reducing SDS-PAGE.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

Shipping with dry ice