protein

PPIE Protein, Human (His)

HY-P71225

10 μg;50 μg

170;510 USD

PPIE Protein, Human (His)

proteins

E. coli

10 μg;50 μg

170;510 USD

The PPIE protein is a key spliceosome component that complexly regulates pre-mRNA splicing through RNA binding and PPIase activity. It prefers single-stranded RNA with polyA and polyU stretches, indicating an affinity for the poly(A) region of the 3'-UTR. PPIE Protein, Human (His) is the recombinant human-derived PPIE protein, expressed by E. coli , with N-6*His labeled tag. The total length of PPIE Protein, Human (His) is 301 a.a., with molecular weight of ~34.0 kDa.

Human

PPIE protein plays a crucial role in pre-mRNA splicing as an integral component of the spliceosome. This multifunctional protein combines RNA-binding and peptidyl-prolyl cis-trans isomerase (PPIase) activities, contributing to the intricate process of splicing regulation. PPIE exhibits a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting its affinity for the poly(A)-region in the 3'-UTR of mRNA molecules. Beyond its role in RNA binding, PPIE catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Notably, it functions as an inhibitor of KMT2A activity, and this regulatory effect is contingent on its proline isomerase activity. The versatile functions of PPIE underscore its significance in coordinating various aspects of cellular processes, including RNA splicing and protein conformational changes.

Peptidyl-Prolyl Cis-Trans Isomerase E; PPIase E; Cyclophilin E; Cyclophilin-33; Rotamase E; PPIE; CYP33

Greater than 95% as determined by reducing SDS-PAGE.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, pH 8.0.

Shipping with dry ice.