protein

IL-5 Protein, Rat

HY-P7102

10 μg;50 μg

115;325 USD

IL-5 Protein, Rat

proteins

E. coli

10 μg;50 μg

115;325 USD

375588571

IL-5 Protein, Rat, a T-cell derived cytokine, stimulates B cell growth and increases immunoglobulin secretion.

Rat

Interleukin-5 (IL-5) is a T-cell derived cytokine which stimulates eosinophil production and activation in human, mice and sheep. IL-5 is active as a growth factor for mouse but not human B cells. The role of IL-5 on ruminant B cells has not been clearly defined. By hybridisation with human IL-5 cDNA, the ovine IL-5 gene is isolated from a liver genomic library. The IL-5 cDNA is obtained by reverse-transcriptase PCR using primers designed from the 5' and 3' coding sequence derived from the ovine IL-5 gene. The sequences of the cDNA shows that there is 79% and 73% nucleotide homology with the human and mouse sequences. The ovine IL-5 cDNA encodes a protein of 132 amino acids and the level of amino acid homology with human and mouse IL-5 is 64% and 56%, respectively. Two cysteine residues are conserved in ovine, human and mouse IL-5. There are two potential N-linked glycoyslation sites in ovine IL-5 [1] .

rRtIL-5; EDF; BCDFII; TRF

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized after extensive dialysis against 20 mM Tris, pH 8.5.

Room temperature in continental US; may vary elsewhere.