protein

SDF-1 alpha/CXCL12 Protein, Human

HY-P70469

2 μg;10 μg;50 μg;250 μg;500 μg;1 mg

52;135;350;890;1250;1750 USD

SDF-1 alpha/CXCL12 Protein, Human

proteins

E. coli

2 μg;10 μg;50 μg;250 μg;500 μg;1 mg

52;135;350;890;1250;1750 USD

The SDF-1 alpha/CXCL12 protein is a chemoattractant for immune cells. SDF-1 alpha/CXCL12 Protein, Human is the recombinant human-derived SDF-1 alpha/CXCL12 protein, expressed by E. coli , with tag free.

Human

SDF-1 alpha/CXCL12 protein functions as a chemoattractant with specific activity on T-lymphocytes and monocytes, excluding neutrophils. Upon activation of the C-X-C chemokine receptor CXCR4, it induces a rapid and transient rise in intracellular calcium ions, facilitating chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) exhibit reduced chemotactic activity, and binding to cell surface proteoglycans appears to inhibit the formation of SDF-1-alpha(3-67), preserving activity at local sites. Additionally, it binds to the atypical chemokine receptor ACKR3, activating the beta-arrestin pathway and serving as a scavenger receptor for SDF-1. Through binding to the allosteric site (site 2) of integrins, it activates ITGAV:ITGB3, ITGA4:ITGB1, and ITGA5:ITGB1 independently of CXCR4. Acting as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase, SDF-1 alpha/CXCL12 stimulates migration of monocytes and T-lymphocytes through CXCR4 and ACKR3, decreasing monocyte adherence to ICAM-1-coated surfaces, a ligand for beta-2 integrins. The SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1-mediated adhesion of monocytes to ICAM-1 through LYN kinase. It inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1, plays a protective role after myocardial infarction, and induces down-regulation and internalization of ACKR3 in various cells. Essential during embryonic development, it is required for B-cell lymphopoiesis, myelopoiesis in bone marrow, and heart ventricular septum formation. Furthermore, SDF-1 alpha/CXCL12 stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7, as well as the growth of stromal cell-dependent pre-B-cells (By similarity). Existing in monomeric or homodimeric forms, the equilibrium is influenced by non-acidic pH, multivalent anions, and binding to CXCR4 or heparin. The monomeric form is vital for full chemotactic activity and resistance to ischemia/reperfusion injury, while the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization without chemotactic activity, serving instead as a selective antagonist that blocks chemotaxis induced by the monomeric form. SDF-1 alpha/CXCL12 interacts with the N-terminus of ACKR3, integrin subunit ITGB3 (via the allosteric site (site 2)), and TNFAIP6 via the Link domain.

Stromal Cell-Derived Factor 1; SDF-1; hSDF-1; C-X-C Motif Chemokine 12; Intercrine Reduced in Hepatomas; IRH; hIRH; Pre-B Cell Growth-Stimulating Factor; PBSF; CXCL12; SDF1; SDF1A; SDF1B

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM PB, 130 mM NaCl, pH 7.3.

Room temperature in continental US; may vary elsewhere.