protein

Flap endonuclease 1/FEN-1 Protein, Human

HY-P70348

5 μg;10 μg;50 μg;100 μg

60;102;290;490 USD

Flap endonuclease 1/FEN-1 Protein, Human

proteins

E. coli

5 μg;10 μg;50 μg;100 μg

60;102;290;490 USD

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease crucial for DNA replication and repair. In replication, it cleaves 5'-overhanging flaps, guiding DNA polymerase and creating a nick for ligation. FEN-1 is integral to the long patch base excision repair pathway, preventing genomic instability. It exhibits 5'-3' exonuclease and RNase H activities, playing roles in rDNA and mitochondrial DNA replication and repair. Flap endonuclease 1/FEN-1 Protein, Human is the recombinant human-derived Flap endonuclease 1/FEN-1 protein, expressed by E. coli , with tag free.

Human

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease with dual enzymatic activities, functioning in both DNA replication and repair processes. In DNA replication, FEN-1 plays a crucial role by cleaving the 5'-overhanging flap structure generated during displacement synthesis, allowing DNA polymerase to navigate the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and tracks to cleave the flap base, creating a nick for subsequent ligation. Additionally, FEN-1 is integral to the long patch base excision repair (LP-BER) pathway, where it cleaves within the apurinic/apyrimidinic (AP) site-terminated flap. Acting as a genome stabilization factor, FEN-1 prevents the equilibration of flaps into structures that may lead to duplications or deletions. Furthermore, the protein exhibits 5'-3' exonuclease activity on nicked or gapped double-stranded DNA and possesses RNase H activity. FEN-1's versatile functions extend to replication and repair processes in rDNA and mitochondrial DNA.

rHuFlap endonuclease 1/FEN-1; Flap Endonuclease 1; FEN-1; DNase IV; Flap Structure-Specific Endonuclease 1; Maturation Factor 1; MF1; hFEN-1; FEN1; RAD2

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 6% trehalose, pH 8.0.

Room temperature in continental US; may vary elsewhere.