protein

UBE1L2/UBA6 Protein, Human (Sf9, His, StrepⅡ)

HY-P701353

10 μg;50 μg

195;550 USD

UBE1L2/UBA6 Protein, Human (Sf9, His, StrepⅡ)

proteins

Sf9 insect cells

10 μg;50 μg

195;550 USD

The ubiquitin E1 enzyme initiates ubiquitination by adenylating ubiquitin with ATP and forming a thioester bond with the E1 enzyme. It exhibits specificity for ubiquitin and excludes ubiquitin-like peptides. UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UBE1L2/UBA6 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is 1051 a.a., .

Human

The Ubiquitin E1 Enzyme initiates the ubiquitination process by activating ubiquitin through a two-step mechanism. It first adenylates the C-terminal glycine residue of ubiquitin with ATP and then forms a thioester bond between this glycine residue and a cysteine residue in the E1 enzyme, releasing free AMP in the process. Notably, it demonstrates specificity for ubiquitin, excluding ubiquitin-like peptides from this activation process. In contrast to UBE1, this enzyme is selective in charging substrate E2 enzymes, excluding cell cycle E2s such as CDC34. Its indispensability is underscored by its role in embryonic development, and it is crucial for the conjugation of UBD/FAT10. Isoform 2 of the enzyme may exert a significant impact on the ubiquitin system, potentially influencing spermatogenesis and male fertility.

UBA6; Ubiquitin-like modifier-activating enzyme 6; Ubiquitin-activating enzyme 6; Monocyte protein 4; MOP-4; Ubiquitin-activating enzyme E1-like protein 2; E1-L2

Greater than 90% as determined by reducing SDS-PAGE.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol.

Shipping with dry ice