protein

TFAM Protein, Human (His)

HY-P701317

20 μg;50 μg

180;400 USD

TFAM Protein, Human (His)

proteins

E. coli

20 μg;50 μg

180;400 USD

TFAM protein regulates lymphangiogenesis, interacts with TEK/TIE2 and ITGA5, and binds to the mitochondrial light chain promoter. It is part of the mitochondrial transcription initiation complex formed with TFB2M and POLRMT and is critical for accurate promoter recognition and transcription initiation. TFAM Protein, Human (His) is the recombinant human-derived TFAM protein, expressed by E. coli , with labeled tag. The total length of TFAM Protein, Human (His) is 204 a.a., with molecular weight of 28.5 kDa.

Human

TFAM protein is involved in the regulation of lymphangiogenesis and interacts with TEK/TIE2 and ITGA5. It binds to the mitochondrial light strand promoter and plays a role in mitochondrial transcription regulation. TFAM is a component of the mitochondrial transcription initiation complex, along with TFB2M and POLRMT, and is required for basal transcription of mitochondrial DNA. TFAM recruits POLRMT to specific promoters and induces structural changes in POLRMT for promoter opening and DNA non-template strand trapping. It is necessary for accurate promoter recognition by the mitochondrial RNA polymerase and promotes transcription initiation. TFAM can unwind DNA and bends the mitochondrial light strand promoter DNA into a U-turn shape through its HMG boxes. It is essential for maintaining normal levels of mitochondrial DNA and may also play a role in organizing and compacting mitochondrial DNA. TFAM can bind DNA as a monomer and can form homodimers. It forms a complex with FOXO3, SIRT3, and POLRMT under metabolic stress and interacts with TFB1M, TFB2M, and CLPX, enhancing DNA-binding.

Transcription factor A, mitochondrial; mtTFA; Testis-specific high mobility group protein (TS-HMG); Hmgts

Greater than 95 % as determined by reducing SDS-PAGE.

Lyophilized from a 0.22 μm filtered solution of 100 mM Tris-HCl, 10 mM Sodium citrate, 2 M NaCl, 6% Trehalose, pH 8.0.

Room temperature in continental US; may vary elsewhere.