protein

APAF1 Protein, Human (P. pastoris, His)

HY-P700502

20 μg;50 μg

140;306 USD

APAF1 Protein, Human (P. pastoris, His)

proteins

P. pastoris

20 μg;50 μg

140;306 USD

APAF1 protein is a key apoptotic component that coordinates cytochrome c-dependent caspase-9 precursor activation and initiates caspase-3 activation and apoptosis. APAF1 forms an apoptotic loop with cytochrome c and dATP, interacts with caspase-9 precursor, and releases mature caspase-9. APAF1 Protein, Human (P. pastoris, His) is the recombinant human-derived APAF1 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of APAF1 Protein, Human (P. pastoris, His) is 312 a.a., with molecular weight of 37.4 kDa.

Human

APAF1 Protein, as an essential component of the apoptosome, orchestrates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), initiating the caspase-3 activation cascade and ultimately leading to apoptosis. The oligomeric form of APAF1 forms a heptameric ring, known as the apoptosome, in the presence of cytochrome c and dATP. Within this complex, APAF1 and pro-caspase-9 interact via their NH2-terminal CARD domains, leading to the release of mature caspase-9. Concurrently, pro-caspase-3 is recruited into the APAF1-pro-caspase-9 complex through interaction with pro-caspase-9, facilitating the activation of caspase-3. Notably, isoform 6 of APAF1 exhibits reduced effectiveness in inducing apoptosis. Additionally, APAF1 interacts with APIP, NAIP/BIRC1, and CIAO2A, contributing to its multifaceted role in the regulation of apoptotic pathways.

APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Room temperature in continental US; may vary elsewhere.