protein

Animal-Free SDF-1 alpha/CXCL12 Protein, Mouse (His)

HY-P700219AF

10 μg;50 μg

290;800 USD

Animal-Free SDF-1 alpha/CXCL12 Protein, Mouse (His)

proteins

E. coli

10 μg;50 μg

290;800 USD

The SDF-1 α/CXCL12 protein acts as a chemoattractant with specific activity on T lymphocytes and monocytes (excluding neutrophils).It activates the CXC chemokine receptor CXCR4, inducing a rapid and transient rise in intracellular calcium ions and promoting chemotaxis.Animal-Free SDF-1 alpha/CXCL12 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeSDF-1 alpha/CXCL12 protein, expressed by E.coli , with C-His labeled tag.This product is for cell culture use only.

Mouse

SDF-1 alpha/CXCL12 protein functions as a chemoattractant specifically active on T-lymphocytes and monocytes, excluding neutrophils. It activates the C-X-C chemokine receptor CXCR4, inducing a rapid and transient rise in intracellular calcium ions and promoting chemotaxis. Additionally, it binds to the atypical chemokine receptor ACKR3, activating the beta-arrestin pathway and acting as a scavenger receptor for SDF-1. Moreover, it binds to the allosteric site (site 2) of integrins, activating ITGAV:ITGB3, ITGA4:ITGB1, and ITGA5:ITGB1 in a CXCR4-independent manner. Acting as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion through the LYN kinase, it stimulates migration of monocytes and T-lymphocytes via CXCR4 and ACKR3, while decreasing monocyte adherence to ICAM-1-coated surfaces. The SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1-mediated adhesion of monocytes to ICAM-1 through LYN kinase. It plays a protective role after myocardial infarction and induces down-regulation and internalization of ACKR3. Crucially, SDF-1 alpha/CXCL12 is indispensable during embryonic development, participating in B-cell lymphopoiesis, myelopoiesis in bone marrow, and heart ventricular septum formation. It stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7, as well as the growth of stromal cell-dependent pre-B-cells. Existing in monomeric or homodimeric forms, the equilibrium is influenced by non-acidic pH, the presence of multivalent anions, and binding to CXCR4 or heparin. The monomeric form is essential for full chemotactic activity and resistance to ischemia/reperfusion injury, while the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization without chemotactic activity, functioning instead as a selective antagonist that blocks chemotaxis induced by the monomeric form. It interacts with the N-terminus of ACKR3, integrin subunit ITGB3 via the allosteric site (site 2), and TNFAIP6 via the Link domain.

CXCL12; Stromal cell-derived factor 1; SDF-1; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; TPAR1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; PBSF; Thymic lymphoma cell-stimulating factor; TLSF; Sdf1

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

Room temperature in continental US; may vary elsewhere.