product summary
company name :
METAFORA biosystems
product type :
protein
product name :
Anti-GLUT1 RBD (receptor binding domain, can function as an antibody)
catalog :
Glut1-G100
quantity :
100 reactions
citations: 9
Reference
Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013;93:611-21 pubmed publisher
Manel N, Battini J, Sitbon M. Human T cell leukemia virus envelope binding and virus entry are mediated by distinct domains of the glucose transporter GLUT1. J Biol Chem. 2005;280:29025-9 pubmed
Loisel-Meyer S, Swainson L, Craveiro M, Oburoglu L, Mongellaz C, Costa C, et al. Glut1-mediated glucose transport regulates HIV infection. Proc Natl Acad Sci U S A. 2012;109:2549-54 pubmed publisher
Kinet S, Swainson L, Lavanya M, Mongellaz C, Montel-Hagen A, Craveiro M, et al. Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells. Retrovirology. 2007;4:31 pubmed
Laval J, Touhami J, Herzenberg L, Conrad C, Taylor N, Battini J, et al. Metabolic adaptation of neutrophils in cystic fibrosis airways involves distinct shifts in nutrient transporter expression. J Immunol. 2013;190:6043-50 pubmed publisher
Oburoglu L, Tardito S, Fritz V, De Barros S, Merida P, Craveiro M, et al. Glucose and glutamine metabolism regulate human hematopoietic stem cell lineage specification. Cell Stem Cell. 2014;15:169-84 pubmed publisher
Montel-Hagen A, Blanc L, Boyer-Clavel M, Jacquet C, Vidal M, Sitbon M, et al. The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis. Blood. 2008;112:4729-38 pubmed publisher
Li J, Cousin C, Tinkler H, Touhami J, Petit V, Thomas N. Profiling of Nutrient Transporter Expression in Human Stem Cell-Derived Cardiomyocytes Exposed to Tyrosine Kinase Inhibitor Anticancer Drugs Using RBD Ligands. J Biomol Screen. 2014;19:1185-1192 pubmed
Hegedus A, Kavanagh Williamson M, Huthoff H. HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells. Retrovirology. 2014;11:98 pubmed publisher
product information
Catalog Number :
Glut1-G100
Product Name :
Anti-GLUT1 RBD (receptor binding domain, can function as an antibody)
Host Species :
No
Clonality :
Recombinant protein
Conjugation :
GFP fusion
Antigen Modification :
No
Product Type :
GFP fusion recombinant protein purified from CHO supernatant
Size :
100 reactions
list of Pubmed id :
25421745;24953180;24809354;23690474;23459372;22308487;18796630;1835881;17504522;15955807;14622599
Image Legend :
Flow cytometry fluorescent shift observed for live non permeabilized human mesenchymal stem cells
Product Description :
GFP fused primary ligand for quantitative assessment of Glut1 / SLC2A1 glucose transporter translocation at the cell surface using flow cytometry or immunoflurescence.
CrossReactivity :
Human, Mouse, Rat, …
ALTnames :
Glut1, GLUT-1, SLC2A1, Glucose Transporter 1
Epitope :
QYVEQLC, Binds to that epitope located within extracellular loop 6 of Glut1 as determined by directed mutagenesis
Localization :
Exclusively binds to the cell surface expression of Glut1 in live non permeabilized cells
Specificity :
No binding observed for following close nutrient transporters from SLC family : ASCT1, ASCT2, RFT1, RFT2, Pit1, Pit2, XPR1, FLVCR1
Purity :
>80%, purified by ion exchange chromatography
Buffer :
Phosphate-buffered solution, pH 7.4
Preservative :
0.1% sodium azide and 20% glycerol
Packaging :
300µL in a 2mL vial shipped in dry ice
Concentration :
0.1 mg/mL
Uses :
Validated for Flow cytometry and immunofluorescence quantitative assessment of Glut1 translocation. Also excellent for cell sorting purposes. Detailed protocols are available in METAFORA website
Application Summary :
IF, FC
Storage :
store in -20degC freezer. Working dilution samples should be discarded if not used within 12 hours.
company information
METAFORA biosystems
4 rue Pierre Fontaine | 91058 Evry cedex
contact@metafora-biosystems.com
www.metafora-biosystems.com
33 (0)1 60 87 89 25
headquarters: France