product summary
company name :
METAFORA biosystems
product type :
protein
product name :
Anti-GLUT1 RBD (receptor binding domain, can function as an antibody)
catalog :
Glut1-G100
quantity :
100 reactions
more info or order :
citations: 9
| Reference |
|---|
Manel N, Battini J, Sitbon M. Human T cell leukemia virus envelope binding and virus entry are mediated by distinct domains of the glucose transporter GLUT1. J Biol Chem. 2005;280:29025-9 pubmed
|
Kinet S, Swainson L, Lavanya M, Mongellaz C, Montel-Hagen A, Craveiro M, et al. Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells. Retrovirology. 2007;4:31 pubmed
|
Li J, Cousin C, Tinkler H, Touhami J, Petit V, Thomas N. Profiling of Nutrient Transporter Expression in Human Stem Cell-Derived Cardiomyocytes Exposed to Tyrosine Kinase Inhibitor Anticancer Drugs Using RBD Ligands. J Biomol Screen. 2014;19:1185-1192 pubmed
|
product information
Catalog Number :
Glut1-G100
Product Name :
Anti-GLUT1 RBD (receptor binding domain, can function as an antibody)
Host Species :
No
Clonality :
Recombinant protein
Conjugation :
GFP fusion
Antigen Modification :
No
Product Type :
GFP fusion recombinant protein purified from CHO supernatant
Size :
100 reactions
list of Pubmed id :
25421745;24953180;24809354;23690474;23459372;22308487;18796630;1835881;17504522;15955807;14622599
Image Legend :
Flow cytometry fluorescent shift observed for live non permeabilized human mesenchymal stem cells
Product Description :
GFP fused primary ligand for quantitative assessment of Glut1 / SLC2A1 glucose transporter translocation at the cell surface using flow cytometry or immunoflurescence.
CrossReactivity :
Human, Mouse, Rat, …
ALTnames :
Glut1, GLUT-1, SLC2A1, Glucose Transporter 1
Epitope :
QYVEQLC, Binds to that epitope located within extracellular loop 6 of Glut1 as determined by directed mutagenesis
Localization :
Exclusively binds to the cell surface expression of Glut1 in live non permeabilized cells
Specificity :
No binding observed for following close nutrient transporters from SLC family : ASCT1, ASCT2, RFT1, RFT2, Pit1, Pit2, XPR1, FLVCR1
Purity :
>80%, purified by ion exchange chromatography
Buffer :
Phosphate-buffered solution, pH 7.4
Preservative :
0.1% sodium azide and 20% glycerol
Packaging :
300µL in a 2mL vial shipped in dry ice
Concentration :
0.1 mg/mL
Uses :
Validated for Flow cytometry and immunofluorescence quantitative assessment of Glut1 translocation. Also excellent for cell sorting purposes. Detailed protocols are available in METAFORA website
Application Summary :
IF, FC
Storage :
store in -20degC freezer. Working dilution samples should be discarded if not used within 12 hours.
more info or order :
company information
METAFORA biosystems
4 rue Pierre Fontaine | 91058 Evry cedex
contact@metafora-biosystems.com
www.metafora-biosystems.com33 (0)1 60 87 89 25
headquarters: France
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