product summary
company name :
MBL International
product type :
antibody
product name :
LC3
catalog :
M152-3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4E+12
reactivity :
human, mouse, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, mass cytometry
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; loading ...; fig 7i
Subramanian M, Hyeon S, Das T, Suh Y, Kim Y, Lee J, et al. UBE4B, a microRNA-9 target gene, promotes autophagy-mediated Tau degradation. Nat Commun. 2021;12:3291 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig s1c
Matsui H, Ito J, Matsui N, Uechi T, Onodera O, Kakita A. Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson's disease. Nat Commun. 2021;12:3101 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1c
Ogasawara Y, Cheng J, Tatematsu T, Uchida M, Murase O, Yoshikawa S, et al. Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets. Nat Commun. 2020;11:4480 pubmed publisher
  • immunocytochemistry; mouse; 1:300; loading ...; fig ev4l
Bera S, Camblor Perujo S, Calleja Barca E, Negrete Hurtado A, Racho J, de Bruyckere E, et al. AP-2 reduces amyloidogenesis by promoting BACE1 trafficking and degradation in neurons. EMBO Rep. 2020;21:e47954 pubmed publisher
  • immunocytochemistry; rhesus macaque; 1:100; loading ...; fig 7a
Du T, Zhu G, Chen Y, Shi L, Liu D, Liu Y, et al. Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys. Aging (Albany NY). 2020;12:6324-6339 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 4c
Boukhalfa A, Nascimbeni A, Ramel D, Dupont N, Hirsch E, Gayral S, et al. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nat Commun. 2020;11:294 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1c
Ye X, Zhu M, Che X, Wang H, Liang X, Wu C, et al. Lipopolysaccharide induces neuroinflammation in microglia by activating the MTOR pathway and downregulating Vps34 to inhibit autophagosome formation. J Neuroinflammation. 2020;17:18 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5c
Zhang Y, Thery F, Wu N, Luhmann E, Dussurget O, Foecke M, et al. The in vivo ISGylome links ISG15 to metabolic pathways and autophagy upon Listeria monocytogenes infection. Nat Commun. 2019;10:5383 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7a
Dang A, Teles R, Weiss D, Parvatiyar K, Sarno E, Ochoa M, et al. IL-26 contributes to host defense against intracellular bacteria. J Clin Invest. 2019;129:1926-1939 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4e
Merrill N, Schipper J, Karnes J, Kauffman A, Martin K, Mackeigan J. PI3K-C2? knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE. 2017;12:e0184909 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1m
Zhao Y, Chen Y, Miao G, Zhao H, Qu W, Li D, et al. The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. Mol Cell. 2017;67:974-989.e6 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1f
Wei J, Xu H, Meng W. Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk. FEBS Lett. 2017;591:2379-2393 pubmed publisher
  • mass cytometry; human; loading ...; fig 1j
  • flow cytometry; human; fig 2f
See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  • flow cytometry; human; loading ...; fig st12
Villani A, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017;356: pubmed publisher
  • flow cytometry; human; fig s6b
Ribeiro C, Sarrami Forooshani R, Setiawan L, Zijlstra Willems E, van Hamme J, Tigchelaar W, et al. Receptor usage dictates HIV-1 restriction by human TRIM5? in dendritic cell subsets. Nature. 2016;540:448-452 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 1
Andersson A, Andersson B, Lorell C, Raffetseder J, Larsson M, Blomgran R. Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages. Sci Rep. 2016;6:28171 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 6b
Follo C, Barbone D, Richards W, Bueno R, Broaddus V. Autophagy initiation correlates with the autophagic flux in 3D models of mesothelioma and with patient outcome. Autophagy. 2016;12:1180-94 pubmed publisher
  • western blot; mouse; loading ...; fig 5a
Tamura Y, Matsunaga Y, Kitaoka Y, Hatta H. Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle. J Gerontol A Biol Sci Med Sci. 2017;72:299-308 pubmed publisher
  • immunocytochemistry; mouse; fig 4
Zhuang H, Tian W, Li W, Zhang X, Wang J, Yang Y, et al. Autophagic Cell Death and Apoptosis Jointly Mediate Cisatracurium Besylate-Induced Cell Injury. Int J Mol Sci. 2016;17:515 pubmed publisher
  • immunocytochemistry; mouse; 1:300; loading ...; fig 2g
  • western blot; mouse; 1:2000; loading ...; fig 2c
Son S, Cha M, Choi H, Kang S, Choi H, Lee M, et al. Insulin-degrading enzyme secretion from astrocytes is mediated by an autophagy-based unconventional secretory pathway in Alzheimer disease. Autophagy. 2016;12:784-800 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 4
Peng Y, Kim M, Hullinger R, O Riordan K, Burger C, Pehar M, et al. Improved proteostasis in the secretory pathway rescues Alzheimer's disease in the mouse. Brain. 2016;139:937-52 pubmed publisher
  • immunocytochemistry; human; 1:100; fig e3
Ketel K, Krauss M, Nicot A, Puchkov D, Wieffer M, Müller R, et al. A phosphoinositide conversion mechanism for exit from endosomes. Nature. 2016;529:408-12 pubmed publisher
  • immunocytochemistry; human; 1:10; fig 2e
Kharaziha P, Chioureas D, Baltatzis G, Fonseca P, Rodriguez P, Gogvadze V, et al. Sorafenib-induced defective autophagy promotes cell death by necroptosis. Oncotarget. 2015;6:37066-82 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2b
Colecchia D, Rossi M, Sasdelli F, Sanzone S, Strambi A, Chiariello M. MAPK15 mediates BCR-ABL1-induced autophagy and regulates oncogene-dependent cell proliferation and tumor formation. Autophagy. 2015;11:1790-802 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1
Moreau K, Ghislat G, Hochfeld W, Renna M, Zavodszky E, Runwal G, et al. Transcriptional regulation of Annexin A2 promotes starvation-induced autophagy. Nat Commun. 2015;6:8045 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s6b
Hirst J, Edgar J, Esteves T, Darios F, Madeo M, Chang J, et al. Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease. Hum Mol Genet. 2015;24:4984-96 pubmed publisher
  • western blot; mouse; fig 1f, 1g
  • western blot; human; fig 1a, 1b
Luo T, Fu J, Xu A, Su B, Ren Y, Li N, et al. PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy. 2016;12:1355-71 pubmed publisher
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Brandstaetter H, Kishi Itakura C, Tumbarello D, Manstein D, Buss F. Loss of functional MYO1C/myosin 1c, a motor protein involved in lipid raft trafficking, disrupts autophagosome-lysosome fusion. Autophagy. 2014;10:2310-23 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
  • immunocytochemistry; human
Li W, Zhang X, Zhuang H, Chen H, Chen Y, Tian W, et al. MicroRNA-137 is a novel hypoxia-responsive microRNA that inhibits mitophagy via regulation of two mitophagy receptors FUNDC1 and NIX. J Biol Chem. 2014;289:10691-701 pubmed publisher
  • western blot; mouse
Liu S, Hartleben B, Kretz O, Wiech T, Igarashi P, Mizushima N, et al. Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury. Autophagy. 2012;8:826-37 pubmed publisher
  • western blot; mouse; 1:2000
Morris C, Stanton M, Manthey K, Oh K, Wagner K. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS ONE. 2012;7:e34308 pubmed publisher
Deitersen J, Berning L, Stuhldreier F, Ceccacci S, Schlütermann D, Friedrich A, et al. High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol. Cell Death Dis. 2021;12:560 pubmed publisher
Arias Fuenzalida J, Jarazo J, Walter J, Gomez Giro G, Forster J, Krueger R, et al. Automated high-throughput high-content autophagy and mitophagy analysis platform. Sci Rep. 2019;9:9455 pubmed publisher
Tian X, Zheng P, Zhou C, Wang X, Ma H, Ma W, et al. DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B. Autophagy. 2019;:1-14 pubmed publisher
Colecchia D, Dapporto F, Tronnolone S, Salvini L, Chiariello M. MAPK15 is part of the ULK complex and controls its activity to regulate early phases of the autophagic process. J Biol Chem. 2018;293:15962-15976 pubmed publisher
Yan M, Ye L, Yin S, Lu X, Liu X, Lu S, et al. Glycycoumarin protects mice against acetaminophen-induced liver injury predominantly via activating sustained autophagy. Br J Pharmacol. 2018;175:3747-3757 pubmed publisher
Kanda R, Hiraike H, Wada Hiraike O, Ichinose T, Nagasaka K, Sasajima Y, et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. BMC Cancer. 2018;18:657 pubmed publisher
Lassak A, Dean M, Wyczechowska D, Wilk A, Marrero L, Trillo Tinoco J, et al. Molecular and Structural Traits of Insulin Receptor Substrate 1/LC3 Nuclear Structures and Their Role in Autophagy Control and Tumor Cell Survival. Mol Cell Biol. 2018;38: pubmed publisher
Newton T, Allison R, Edgar J, Lumb J, Rodger C, Manna P, et al. Mechanistic basis of an epistatic interaction reducing age at onset in hereditary spastic paraplegia. Brain. 2018;141:1286-1299 pubmed publisher
Colecchia D, Stasi M, Leonardi M, Manganelli F, Nolano M, Veneziani B, et al. Alterations of autophagy in the peripheral neuropathy Charcot-Marie-Tooth type 2B. Autophagy. 2017;: pubmed publisher
Chang M, Srinivasan K, Friedman B, Suto E, Modrusan Z, Lee W, et al. Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation. J Exp Med. 2017;214:2611-2628 pubmed publisher
Aizawa S, Fujiwara Y, Contu V, Hase K, Takahashi M, Kikuchi H, et al. Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes. Autophagy. 2016;12:565-78 pubmed publisher
company information
MBL International
15A Constitution Way Woburn, MA 01801
customerservice@mblintl.com
http://www.mblintl.com
headquarters: United States