Human IFN-gamma/Granzyme B FluoroSpotPLUS kit | MABTECH FSP-0110-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MABTECH

product type :

ELISA/assay

product name :

Human IFN-gamma/Granzyme B FluoroSpotPLUS kit

catalog :

FSP-0110-2

quantity :

2 plates

price :

770 USD

citations: 13

Reference
Liebowitz D, Gottlieb K, Kolhatkar N, Garg S, Asher J, Nazareno J, et al. Efficacy, immunogenicity, and safety of an oral influenza vaccine: a placebo-controlled and active-controlled phase 2 human challenge study. Lancet Infect Dis. 2020;20:435-444 pubmed publisher
Bronge M, Ruhrmann S, Carvalho Queiroz C, Nilsson O, Kaiser A, Holmgren E, et al. Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. J Autoimmun. 2019;: pubmed publisher
Jelcić I, Al Nimer F, Wang J, Lentsch V, Planas R, Jelcic I, et al. Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis. Cell. 2018;175:85-100.e23 pubmed publisher
Fostier K, Caers J, Meuleman N, Broos K, Corthals J, Thielemans K, et al. Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. 2018;9:20476-20489 pubmed publisher
Lund G, Brand S, Ramos T, Jimeno L, Boissy P, Vega F, et al. Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. 2018;73:1013-1021 pubmed publisher
Jackson S, Sedikides G, Mason G, Okecha G, Wills M. Human Cytomegalovirus (HCMV)-Specific CD4+ T Cells Are Polyfunctional and Can Respond to HCMV-Infected Dendritic Cells In Vitro. J Virol. 2017;91: pubmed publisher
Körber N, Behrends U, Hapfelmeier A, Protzer U, Bauer T. Validation of an IFN?/IL2 FluoroSpot assay for clinical trial monitoring. J Transl Med. 2016;14:175 pubmed publisher
Kaiser Y, Lepzien R, Kullberg S, Eklund A, Smed SÃ¶rensen A, Grunewald J. Expanded lung T-bet+RORÎ³T+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. 2016;48:484-94 pubmed publisher
Weinberg A, Muresan P, Richardson K, Fenton T, Domínguez T, Bloom A, et al. Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. PLoS ONE. 2015;10:e0122431 pubmed publisher
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed publisher
Han W, van Twillert I, Poelen M, Helm K, van de Kassteele J, Verheij T, et al. Loss of multi-epitope specificity in memory CD4(+) T cell responses to B. pertussis with age. PLoS ONE. 2013;8:e83583 pubmed publisher
Sridhar S, Begom S, Bermingham A, Hoschler K, Adamson W, Carman W, et al. Cellular immune correlates of protection against symptomatic pandemic influenza. Nat Med. 2013;19:1305-12 pubmed publisher
Casey R, Blumenkrantz D, Millington K, Montamat Sicotte D, Kon O, Wickremasinghe M, et al. Enumeration of functional T-cell subsets by fluorescence-immunospot defines signatures of pathogen burden in tuberculosis. PLoS ONE. 2010;5:e15619 pubmed publisher

product information

Product Code :

FSP-0110-2

Product Name :

Human IFN-gamma/Granzyme B FluoroSpotPLUS kit

Categories :

FluoroSpot Kits

Size :

2 plates

Size Number :

Size Unit :

plates

EUR :

650

USD :

770 USD

GBP :

575

AUD :

1050

SEK :

6800

Format :

pre-coated plates

Cell Type :

T cells

Application :

FluoroSpot

Reactivities :

human, human

Analytes :

IFNG, Granzyme B, Granzyme B, IFNG

Recommended Products :

FSP-011009-2

Contents :

BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (GB11),Anti-BAM-490,SA-550,anti-CD3 mAb,Anti-CD28 mAb (CD28-A),Fluorescence enhancer,Pre-coated FluoroSpot plates (mAb 1-D1K and GB10)

Product Header :

FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and Granzyme B

Description 1 :

The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/Granzyme B FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

Description 2 :

Pre-coated plates enable a more rapid assay with minimized variability.

References :

Jelcic, I., et al. (2018). Memory B Cells Activate Brain-Homing, Autoreactive CD4(+) T Cells in Multiple Sclerosis. Cell. (http://www.ncbi.nlm.nih.gov/pubmed/30173916) Bronge, M., et al. (2019). Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. Journal of autoimmunity. (http://www.ncbi.nlm.nih.gov/pubmed/31054941) Jackson, S. E., et al. (2017). HCMV Specific CD4+ T Cells are poly-functional and can respond to HCMV Infected Dendritic Cells in vitro. Journal of virology. (http://www.ncbi.nlm.nih.gov/pubmed/28053099) Fostier, K., et al. (2018). Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. (http://www.ncbi.nlm.nih.gov/pubmed/29755666) Liebowitz, D., et al. (2020). Efficacy, immunogenicity, and safety of an oral influenza vaccine: a placebo-controlled and active-controlled phase 2 human challenge study. Lancet Infect Dis. (http://www.ncbi.nlm.nih.gov/pubmed/31978354)

Shelf Life :

12 months

Last updated :

2013-07-01