Human IFN-gamma/Granzyme B FluoroSpotPLUS kit | MABTECH FSP-0110-10 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MABTECH

product type :

ELISA/assay

product name :

Human IFN-gamma/Granzyme B FluoroSpotPLUS kit

catalog :

FSP-0110-10

quantity :

10 plates

price :

3360 USD

citations: 13

Reference
Bronge M, Ruhrmann S, Carvalho Queiroz C, Nilsson O, Kaiser A, Holmgren E, et al. Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. J Autoimmun. 2019;: pubmed publisher
Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed publisher
Schultz H, Østergaard S, Sidney J, Lamberth K, Sette A. The effect of acylation with fatty acids and other modifications on HLA class II:peptide binding and T cell stimulation for three model peptides. PLoS ONE. 2018;13:e0197407 pubmed publisher
Danielzik T, Koldehoff M, Buttkereit U, Beelen D, Horn P, Lindemann M. Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. 2018;59:490-492 pubmed publisher
Jackson S, Sedikides G, Mason G, Okecha G, Wills M. Human Cytomegalovirus (HCMV)-Specific CD4+ T Cells Are Polyfunctional and Can Respond to HCMV-Infected Dendritic Cells In Vitro. J Virol. 2017;91: pubmed publisher
Körber N, Behrends U, Hapfelmeier A, Protzer U, Bauer T. Validation of an IFN?/IL2 FluoroSpot assay for clinical trial monitoring. J Transl Med. 2016;14:175 pubmed publisher
Kaiser Y, Lepzien R, Kullberg S, Eklund A, Smed SÃ¶rensen A, Grunewald J. Expanded lung T-bet+RORÎ³T+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. 2016;48:484-94 pubmed publisher
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed publisher
Brunskole Hummel I, Huber B, Wenzel J, Jilg W. Markers of Protection in Children and Adolescents Six to Fourteen Years After Primary Hepatitis B Vaccination in Real Life: A Pilot Study. Pediatr Infect Dis J. 2016;35:286-91 pubmed publisher
Dillenbeck T, Gelius E, Fohlstedt J, Ahlborg N. Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-Î³, IL-17A and IL-22 Responses. Cells. 2014;3:1116-30 pubmed publisher
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed publisher
Chauvat A, Benhamouda N, Gey A, Lemoine F, Paulie S, Carrat F, et al. Clinical validation of IFN?/IL-10 and IFN?/IL-2 FluoroSpot assays for the detection of Tr1 T cells and influenza vaccine monitoring in humans. Hum Vaccin Immunother. 2014;10:104-13 pubmed publisher
Ohrmalm L, Smedman C, Wong M, Broliden K, Tolfvenstam T, Norbeck O. Decreased functional T lymphocyte-mediated cytokine responses in patients with chemotherapy-induced neutropenia. J Intern Med. 2013;274:363-70 pubmed publisher

product information

Product Code :

FSP-0110-10

Product Name :

Human IFN-gamma/Granzyme B FluoroSpotPLUS kit

Categories :

FluoroSpot Kits

Size :

10 plates

Size Number :

Size Unit :

plates

EUR :

2900

USD :

3360 USD

GBP :

2530

AUD :

4570

SEK :

29000

Format :

pre-coated plates

Cell Type :

T cells

Application :

FluoroSpot

Reactivities :

human, human

Analytes :

IFNG, Granzyme B, Granzyme B, IFNG

Recommended Products :

FSP-011009-10

Contents :

BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (GB11),Anti-BAM-490,SA-550,anti-CD3 mAb,Anti-CD28 mAb (CD28-A),Fluorescence enhancer,Pre-coated FluoroSpot plates (mAb 1-D1K and GB10)

Product Header :

FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and Granzyme B

Description 1 :

The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/Granzyme B FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

Description 2 :

Pre-coated plates enable a more rapid assay with minimized variability.

References :

Schultz, H. S., et al. (2018). The effect of acylation with fatty acids and other modifications on HLA class II:peptide binding and T cell stimulation for three model peptides. PLoS ONE. (http://www.ncbi.nlm.nih.gov/pubmed/29758051) Dillenbeck, T., et al. (2014). Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-gamma, IL-17A and IL-22 Responses. Cells. (http://www.ncbi.nlm.nih.gov/pubmed/25437441) Bronge, M., et al. (2019). Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. Journal of autoimmunity. (http://www.ncbi.nlm.nih.gov/pubmed/31054941) Danielzik, T., et al. (2017). Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. (http://www.ncbi.nlm.nih.gov/pubmed/28573960) Levin, M. J., et al. (2018). Th1 memory differentiates recombinant from live herpes zoster vaccines. The Journal of clinical investigation. (http://www.ncbi.nlm.nih.gov/pubmed/30024861)

Shelf Life :

12 months

Last updated :

2013-07-01