CNOT8 Antibody (aa227-255) LS-C99242 | LifeSpan Biosciences LS-C99242 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

CNOT8 Antibody (aa227-255) LS-C99242

catalog :

LS-C99242

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Yi H, Park J, Ha M, Lim J, Chang H, Kim V. PABP Cooperates with the CCR4-NOT Complex to Promote mRNA Deadenylation and Block Precocious Decay. Mol Cell. 2018;70:1081-1088.e5 pubmed publisher

image

image 1 :

CNOT8 Antibody western blot of U251 cell line lysates (35 ug/lane). The CNOT8 antibody detected the CNOT8 protein (arrow).

image 2 :

Fluorescent confocal image of HeLa cell stained with CNOT8 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.

image 3 :

Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

AntibodyID :

100814

AntibodyName :

LS-C99242

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

CNOT8 Antibody (aa227-255) LS-C99242

Specificity :

This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa227-255

PresentationDesc :

PBS, 0.09% Sodium Azide

PurificationDesc :

Protein A purified

RecommendedStorageDesc :

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Gene :

CNOT8

BlockingPeptide :

LS-E8258

StandardGeneSymbol :

CNOT8

Reactivity :

Human

Usage :

Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC, IHC-P (1:10 - 1:50), WB (1:1000)

UsageNotRecommended :

IHC-P

ShortWebDescription :

CNOT8 antibody LS-C99242 is an unconjugated rabbit polyclonal antibody to human CNOT8 (aa227-255). Validated for Flow, IF, IHC and WB. Cited in 1 publication.

Synonyms :

CNOT8, CAF1-like protein, CALIFp, CCR4-associated factor 8, CAF2, HCAF1, POP2, CAF1, CALIF

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.