This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Calprotectin Antibody (clone 27E10) LS-C96223
catalog :
LS-C96223
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
27E10
application :
immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
97710
AntibodyName :
LS-C96223
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Calprotectin Antibody (clone 27E10) LS-C96223
Specificity :
The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80% of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes; peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.
ClonalityDesc :
Monoclonal
CloneName :
27E10
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS pH 7.2, 5mg/ml BSA, and 0.05% Kathon CG
ImmunogenDesc :
Cultured human monocytes
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
ResuspensionLiquidDesc :
Reconstitute with 0.5ml distilled H2O.
Gene :
Calprotectin
Reactivity :
Bovine, Human, Rhesus monkey
NonReactiveSpecies :
Porcine
Usage :
ELISA, IHC, IHC-P (1 µg/ml)
UsageNotRecommended :
IHC-Fr
ShortWebDescription :
Calprotectin antibody LS-C96223 is an unconjugated mouse monoclonal antibody to Calprotectin from human. It is reactive with human, bovine and rhesus monkey. Validated for ELISA and IHC.
UsageText :
For IHC-P, Proteinase K pretreatment is recommended for antigen retrieval.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.