This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PCNA Antibody LS-C9526
catalog :
LS-C9526
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
10267
AntibodyName :
LS-C9526
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PCNA Antibody LS-C9526
Specificity :
Recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells, proliferating cells in solid tumors, and blast cells in leukemia patients. Species cross-reactivity: Invertebrates and vertebrates.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.2, 1 mg/ml BSA, 0.1% Sodium Azide
ImmunogenDesc :
Rat PCNA made in the protein A vector of pR1T2T.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG2a
Gene :
PCNA
StandardGeneSymbol :
PCNA
Reactivity :
Human
Usage :
Flo, IF, IHC, IHC-P, IP, WB (1:250 - 1:1000)
ShortWebDescription :
PCNA antibody LS-C9526 is an unconjugated mouse monoclonal antibody to human PCNA. Validated for Flow, IF, IHC, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Indirect Flow Cytometry. Western Blot: 1:250-1:1000, Nuclear extracts are preferred over whole cell protein extracts. Detects a polypeptide at 36kD with an isoelectric point of 4.8. Immunohistochemistry (paraffin embedded tissue sections only): 1:20-1:200, may be used on formalin (buffered or unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air drying overnight on poly-L-lysine coated slides is recommended. Incubate for 1 hour at 25?C using standard ABC. Positive control: Tonsil or reactive lymph node.
Synonyms :
PCNA, Cyclin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.