This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PARP1 Antibody LS-C9486
catalog :
LS-C9486
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, bovine
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
product information
AntibodyID :
10227
AntibodyName :
LS-C9486
TargetSpecies :
Bovine
Host Species :
Mouse
Product Name :
PARP1 Antibody LS-C9486
Specificity :
Recognizes human and bovine PARP-1. The epitope is in the zinc finger II DNA binding domain of PARP-1. Detects bands of ~116kD (intact PARP-1) and ~24kD (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with mouse or rat PARP-1.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).
PurificationDesc :
Ascites
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
PARP1
StandardGeneSymbol :
PARP1
Reactivity :
Bovine, Human
Usage :
ELISA, Func, ICC (1:500), IP (1:50), WB (1:5000)
ShortWebDescription :
PARP1 antibody LS-C9486 is an unconjugated mouse monoclonal antibody to PARP1 from bovine. It is reactive with human and bovine. Validated for ELISA, Func, ICC, IP and WB.
UsageText :
ELISA: >1:200000. Dilution buffer: PBS, pH 7.4, containing 1% non fat dried milk and 0.05% Tween 20. Detectable antigen range: 50 ng of PARP-1. Immunocytochemistry: 1:500. Dilution buffer: 50 mM TRIS, pH 7.5, containing 150 mM NaCl and 5% milk. Immunoprecipitation: 1:50. Dilution buffer: RiPA buffer Protein G-Sepharose. Western Blot: 1:5000. See also separate Western blot protocol. Functional Application: Neutralizes the biological activity of PARP (~80%).
Synonyms :
PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.