This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
POU5F1 / OCT4 Antibody (aa282-297) LS-C9379
catalog :
LS-C9379
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
product information
AntibodyID :
10120
AntibodyName :
LS-C9379
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
POU5F1 / OCT4 Antibody (aa282-297) LS-C9379
Specificity :
Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa282-297
PresentationDesc :
PBS, pH 7.4, No preservatives added
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide (aa 282-297) of Oct4.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
POU5F1 / OCT4
StandardGeneSymbol :
POU5F1
Reactivity :
Rodent, Human
Usage :
ICC (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:200 - 1:1000)
ShortWebDescription :
OCT4 antibody LS-C9379 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) (aa282-297) from human. It is reactive with human and rodent. Validated for ICC, IHC and WB.
UsageText :
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20ºC. Alternative is fixation with 4% PFA and treatment with 0.05% trypsin solution (incubation 10 min. at 37ºC, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.
Synonyms :
POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3, OCT3/4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.