This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IRS1 Antibody (aa1222-1235, Biotin) LS-C8283
catalog :
LS-C8283
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
reactivity :
human, rat
application :
western blot, immunoprecipitation
product information
AntibodyID :
9024
AntibodyName :
LS-C8283
TargetSpecies :
Rat
Host Species :
Rabbit
Product Name :
IRS1 Antibody (aa1222-1235, Biotin) LS-C8283
Specificity :
Recognizes rat IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Biotin
AntigenModification :
aa1222-1235
PresentationDesc :
PBS, pH 7.4, 0.05% Sodium Azide, 40% Glycerol
ImmunogenDesc :
Carboxy terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%); Human, Gorilla, Mouse, Hamster, Rabbit (93%); Marmoset, Chicken (86%).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
IRS1
StandardGeneSymbol :
IRS1
Reactivity :
Rat
Usage :
IP, WB (0.5 - 2 µg/ml)
ShortWebDescription :
IRS1 antibody LS-C8283 is a biotin-conjugated rabbit polyclonal antibody to rat IRS1 (aa1222-1235). Validated for IP and WB.
UsageText :
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects IRS-1 in RIPA lysates from 3T3/A31 mouse fibroblasts. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRS-1 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 ug/ml. Immunoprecipitation: 4 ug immunoprecipitates IRS-1 from 500 ug of 3T3/A31 RIPA lysate. Positive control: 3T3/A31 cell lysate. Load 20 ug of reduced lysate per lane for minigels. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.