product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
Collagen I Antibody (C-Terminus) LS-C79516
catalog :
LS-C79516
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Isgrò M, Bianchetti L, Marini M, Bellini A, Schmidt M, Mattoli S. The C-C motif chemokine ligands CCL5, CCL11, and CCL24 induce the migration of circulating fibrocytes from patients with severe asthma. Mucosal Immunol. 2013;6:718-27 pubmed publisher
product information
AntibodyID :
80142
AntibodyName :
LS-C79516
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Collagen I Antibody (C-Terminus) LS-C79516
Specificity :
Recognizes Collagen Type I near the C-terminal. Displays a high affinity for human, bovine and sheep Type I Collagens. There is no evidence for cross-reactivity with Collagen Types III, V and VI or connective tissue proteins (Elastin, Fibronectin and Laminin) at suggested working concentrations. Species cross-reactivity: Human bovine, sheep, porcine and canine. Does not react with mouse, rat, guinea pig, squirrel, equine, kangaroo or chicken.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
50 mM Tris, pH 4.5, 150 mM NaCl, 0.1% Bronidox
ImmunogenType :
Purified protein
ImmunogenDesc :
Human collagen.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
Collagen I
Reactivity :
Sheep, Human
Usage :
ELISA (1:50 - 1:250), Flo (1:100), IHC, IHC-Fr, WB
ShortWebDescription :
Collagen I antibody LS-C79516 is an unconjugated mouse monoclonal antibody to Collagen I (C-Terminus) from human. It is reactive with human and sheep. Validated for ELISA, Flow, IHC and WB. Cited in 1 publication.
UsageText :
Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry, and Western Blot. ELISA (Capture): 2-4 ug/ml. ELISA (Detection): 10 ug/ml will detect 50 ng of human Collagen Type I with no reactivity with other Collagens. Higher levels of antibody may be necessary to detect lower concentrations of Collagen. Immunohistochemistry (Unfixed frozen sections): 1:50-1:250. Flow Cytometry: 1:100. Western Blot (non-denatured and non-reduced): 1:500. Positive control: Human kidney lysate.
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.