This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CCR7 Antibody (clone 4B12) LS-C78053
catalog :
LS-C78053
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
4B12

The same clone is also sold as:
reactivity :
human, mouse
application :
immunohistochemistry, neutralization, flow cytometry
product information
AntibodyID :
78679
AntibodyName :
LS-C78053
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
CCR7 Antibody (clone 4B12) LS-C78053
Specificity :
Recognizes mouse CCR7.
ClonalityDesc :
Monoclonal
CloneName :
4B12
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.4, 5% Trehalose
ImmunogenType :
Cells
ImmunogenDesc :
RBC-2H3 cells expressing mCCR7.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS.
IsotypeName :
IgG2a
Gene :
CCR7
StandardGeneSymbol :
CCR7
gene family :
GPCR
Subfamily :
Chemokine
Reactivity :
Mouse
Usage :
Flo, Func, IHC (25 µg/ml), Neut
ShortWebDescription :
CCR7 antibody LS-C78053 is an unconjugated rat monoclonal antibody to mouse CCR7. Validated for Flow, Func, IHC and Neut.
UsageText :
Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 ug/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 ug/mL in the presence of 25 ng/mL of rmMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.
Synonyms :
CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.