This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CPA2 Antibody LS-C77719
catalog :
LS-C77719
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, neutralization, immunoprecipitation
product information
AntibodyID :
78345
AntibodyName :
LS-C77719
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CPA2 Antibody LS-C77719
Specificity :
Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5%, canine 90%, rat 87%, mouse 86% and chicken 70%.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
NS0-derived recombinant human rhCPA2.
PurificationDesc :
Protein G purified
ResuspensionLiquidDesc :
Phosphate Buffered Saline (PBS)
IsotypeName :
IgG2b
Gene :
CPA2
StandardGeneSymbol :
CPA2
gene family :
Exopeptidase
Subfamily :
Metallopeptidase M14
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), IP (25 µg/ml), Neut, WB (1 - 2 µg/ml)
ShortWebDescription :
CPA2 antibody LS-C77719 is an unconjugated mouse monoclonal antibody to human CPA2. Validated for ELISA, IP, Neut and WB.
UsageText :
Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Western blot:1-2 ug/ml the detection limit is ~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold. Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50% of the enzymatic activity was inhibited at ~1.5 ug/ml (IC50) under conditions in which the enzyme was. present at 0.1 ug/ml and the substrate concentration was 0.1 mM. Considering the. molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at. approximately 5:1 molar ratio of the antibody to the enzyme. Immunoprecipitation: 25 ug/ml. Direct ELISA: 0.5-1.0 ug/mL.
Synonyms :
CPA2, Carboxypeptidase A2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.