This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
West Nile Virus Antibody (clone 345712) LS-C76863
catalog :
LS-C76863
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
345712
application :
western blot, ELISA, neutralization, immunoprecipitation
product information
AntibodyID :
77489
AntibodyName :
LS-C76863
TargetSpecies :
West Nile Virus
Host Species :
Mouse
Product Name :
West Nile Virus Antibody (clone 345712) LS-C76863
Specificity :
Recognizes West Nile Virus NS2b subunit.
ClonalityDesc :
Monoclonal
CloneName :
345712
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from TBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
E. coli-derived rwnvNS3/NS2b.
PurificationDesc :
Protein G purified
ResuspensionLiquidDesc :
Sterile TBS
IsotypeName :
IgG3
Gene :
West Nile Virus
Reactivity :
West Nile Virus
Usage :
ELISA, IP, Neut, WB
ShortWebDescription :
West Nile Virus antibody LS-C76863 is an unconjugated mouse monoclonal antibody to west nile virus West Nile Virus. Validated for ELISA, IP, Neut and WB.
UsageText :
Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50% of the proteolytic activity was inhibited by the antibody at approximately 13 ug/mL (IC50) under conditions in which the enzyme was present at 10 ug/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 ug/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.