product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
SMAD1 Antibody LS-C75853
catalog :
LS-C75853
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
LifeSpan Biosciences product webpage
citations: 5
Published Application/Species/Sample/DilutionReference
  • western blot; human
Li X, Rhee D, Malhotra R, Mayeur C, Hurst L, Ager E, et al. Progesterone receptor membrane component-1 regulates hepcidin biosynthesis. J Clin Invest. 2016;126:389-401 pubmed publisher
  • western blot; mouse; fig 5
Malhotra R, Burke M, Martyn T, Shakartzi H, Thayer T, O Rourke C, et al. Inhibition of bone morphogenetic protein signal transduction prevents the medial vascular calcification associated with matrix Gla protein deficiency. PLoS ONE. 2015;10:e0117098 pubmed publisher
Zhang H, Du L, Zhong Y, Flanders K, Roberts J. Transforming growth factor-? stimulates Smad1/5 signaling in pulmonary artery smooth muscle cells and fibroblasts of the newborn mouse through ALK1. Am J Physiol Lung Cell Mol Physiol. 2017;313:L615-L627 pubmed publisher
Shahid M, Spagnolli E, Ernande L, Thoonen R, Kolodziej S, Leyton P, et al. BMP type I receptor ALK2 is required for angiotensin II-induced cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2016;310:H984-94 pubmed publisher
Mayeur C, Lohmeyer L, Leyton P, Kao S, Pappas A, Kolodziej S, et al. The type I BMP receptor Alk3 is required for the induction of hepatic hepcidin gene expression by interleukin-6. Blood. 2014;123:2261-8 pubmed publisher
product information
AntibodyID :
76479
AntibodyName :
LS-C75853
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
SMAD1 Antibody LS-C75853
Specificity :
Recognizes human Smad1.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.05% Sodium Azide, 1% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human Smad1.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
SMAD1
StandardGeneSymbol :
SMAD1
Reactivity :
Mouse, Rat, Human
Usage :
Flo (0.5 - 2 µg/ml), ICC (2 - 100 µg/ml), IP, WB (0.2 - 2 µg/ml)
UsageNotRecommended :
IHC-P
ShortWebDescription :
SMAD1 antibody LS-C75853 is an unconjugated mouse monoclonal antibody to SMAD1 from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IP and WB. Cited in 4 publications.
UsageText :
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Flow Cytometry: 0.5-2 ug/ml. HeLa cells were fixed with 2% paraformaldehyde in 90% methanol. Western Blot: 0.2-2 ug/ml. Immunocytochemistry: 2-100 ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Immunoprecipitation: 100-500 ug/sample.
Synonyms :
SMAD1, BSP1, BSP-1, JV4-1, MADH1, Mothers against DPP homolog 1, JV41, SMAD family member 1, TGF-beta signaling protein 1, MADR1, HSMAD1, MAD homolog 1, Mad-related protein 1, SMAD 1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.