This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
RET Antibody LS-C75422
catalog :
LS-C75422
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, flow cytometry
product information
AntibodyID :
76048
AntibodyName :
LS-C75422
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
RET Antibody LS-C75422
Specificity :
Recognizes human Ret. Species cross-reactivity: Mouse.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.4, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS to 0.5 mg/ml.
IsotypeName :
IgG1
Gene :
RET
StandardGeneSymbol :
RET
gene family :
Protein Kinase
Subfamily :
Cadherin
Reactivity :
Mouse, Human
Usage :
ELISA (0.5 - 1 µg/ml), Flo (50 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
RET antibody LS-C75422 is an unconjugated mouse monoclonal antibody to RET from human. It is reactive with human and mouse. Validated for ELISA, Flow and WB.
UsageText :
Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 ug/ml; For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Western Blot: 1-2 ug/ml; Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 ug/ml; The detection limit for rhRet and rmRet is ~6 ng/well.
Synonyms :
RET, Cadherin family member 12, CDHF12, C-ret, Hirschsprung disease 1, HSCR1, Hydroxyaryl-protein kinase, Oncogene ret, Proto-oncogene c-Ret, RET-ELE1, RET51, Ret proto-oncogene, RET transforming sequence, RET43, Men2-ret, MEN2B, MTC1, Receptor tyrosine kinase, CDHR16, MEN2A, PTC, RET9
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.