This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PODXL / Podocalyxin Antibody (Extracellular Domain) LS-C75196
catalog :
LS-C75196
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry, flow cytometry
product information
AntibodyID :
75822
AntibodyName :
LS-C75196
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PODXL / Podocalyxin Antibody (Extracellular Domain) LS-C75196
Specificity :
Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
Extracellular Domain
PresentationDesc :
Lyophilized from PBS, pH 7.4, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
NS0-derived rhPodocalyxin extracellular domain.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in 200 ul sterile PBS
IsotypeName :
IgG2a
Gene :
PODXL / Podocalyxin
StandardGeneSymbol :
PODXL
Reactivity :
Human
Usage :
Flo, ICC (10 µg/ml), WB
ShortWebDescription :
Podocalyxin antibody LS-C75196 is an unconjugated mouse monoclonal antibody to human Podocalyxin (PODXL) (Extracellular Domain). Validated for Flow, ICC and WB.
UsageText :
Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 ug/ml using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. Antibody binding may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Synonyms :
PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.