This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Phosphotyrosine Antibody LS-C74885
catalog :
LS-C74885
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
western blot, immunoprecipitation
product information
AntibodyID :
75511
AntibodyName :
LS-C74885
Host Species :
Mouse
Product Name :
Phosphotyrosine Antibody LS-C74885
Specificity :
PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no cross-reactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective. Species cross-reactivity: It is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosine phosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 40% glycerol, 0.1% proclin.
ImmunogenType :
Hapten
ImmunogenDesc :
Tyrosine-phosphorylated proteins.
PurificationDesc :
Immunoaffinity purified
IsotypeName :
IgG1,k
Gene :
Phosphotyrosine
Usage :
IP, WB (1:500)
ShortWebDescription :
Phosphotyrosine antibody LS-C74885 is an unconjugated mouse monoclonal antibody to Phosphotyrosine. Validated for IP and WB.
UsageText :
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 1:500 to 1:1000. Immunoprecipitation:.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.