This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
H2AFX / H2AX Antibody (phospho-Ser139) LS-C7424
catalog :
LS-C7424
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation
product information
AntibodyID :
8165
AntibodyName :
LS-C7424
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
H2AFX / H2AX Antibody (phospho-Ser139) LS-C7424
Specificity :
Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer139
PresentationDesc :
PBS, pH 7.6, 0.07% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
Synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
H2AFX / H2AX
StandardGeneSymbol :
H2AFX
Reactivity :
Rabbit, Dog, Bovine, Human, Monkey
Usage :
ICC (2 µg/ml), IF, IP, WB
ShortWebDescription :
H2AX antibody LS-C7424 is an unconjugated mouse monoclonal antibody to H2AX (H2AFX) (pSer139) from human. It is reactive with human, bovine, dog and other species. Validated for ICC, IF, IP and WB.
UsageText :
Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 ug/ml detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue.
Synonyms :
H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.