This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PD-L2 / PDCD1LG2 / CD273 Antibody LS-C74220
catalog :
LS-C74220
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, flow cytometry
product information
AntibodyID :
74846
AntibodyName :
LS-C74220
TargetSpecies :
Mouse
Host Species :
Goat
Product Name :
PD-L2 / PDCD1LG2 / CD273 Antibody LS-C74220
Specificity :
Recognizes mouse PD-L2. In direct ELISAs and Western Blots, this antibody shows ~20% cross-reactivity with rhPD-L2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
NS0-derived rmPD-L2.
PurificationDesc :
Immunoaffinity purified
ResuspensionLiquidDesc :
Phosphate Buffered Saline (PBS)
IsotypeName :
IgG
Gene :
PD-L2 / PDCD1LG2 / CD273
StandardGeneSymbol :
PDCD1LG2
Reactivity :
Mouse
Usage :
ELISA, Flo, Func, IHC, WB
ShortWebDescription :
CD273 antibody LS-C74220 is an unconjugated goat polyclonal antibody to mouse CD273 (PD-L2 / PDCD1LG2). Validated for ELISA, Flow, Func, IHC and WB.
UsageText :
Western Blot, Immunohistochemistry, Flow Cytometry, Blocking of receptor-ligand interaction, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-L2 in cells and tissues. The working dilution is 2-15 ug/mL. Flow Cytometry: This antibody has been tested on Tim-1 stimulated RAW 264.7 cells for use in flow cytometry. Dilute this antibody to 50 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Blocking of receptor-ligand interaction: Approximately 1-4 ug/mL of this antibody will block 50% of the binding of 1 ug/mL of rmPD-1 to immobilized rmPD-L2/Fc chimera (100 ul of a 1 ug/mL solution was coated in each well) in a functional ELISA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 1.2 ng/well.
Synonyms :
PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.