This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NOTCH1 Antibody (aa2428-2556) LS-C73692
catalog :
LS-C73692
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
74318
AntibodyName :
LS-C73692
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
NOTCH1 Antibody (aa2428-2556) LS-C73692
Specificity :
Recognizes human Notch-1 IC.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa2428-2556
PresentationDesc :
Lyophilized PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
E. coli-derived rhNotch-1 IC. (aa 2428 - 2556).
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile PBS.
IsotypeName :
IgG2a
Gene :
NOTCH1
StandardGeneSymbol :
NOTCH1
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), ICC (10 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
NOTCH1 antibody LS-C73692 is an unconjugated mouse monoclonal antibody to human NOTCH1 (aa2428-2556). Validated for ELISA, ICC and WB.
UsageText :
Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Direct ELISA: 0.5-1.0 ug/ml detection limit for rhNotch-1 IC is ~3 ng/well. Western Blot: 1-2 ug/ml detection limit for rhNotch-1 IC is ~25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml. For SAOS2 osteosarcoma cells, cells were fixed with PBS containing 4% paraformaldehyde for 20min at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA for 45 min at RT. After blocking, cells were incubated with primary antibody overnight at 4?C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Synonyms :
NOTCH1, Notch 1, TAN1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.