This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Myc Tag Antibody (aa410-420) LS-C72945
catalog :
LS-C72945
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
western blot, immunoprecipitation
product information
AntibodyID :
73571
AntibodyName :
LS-C72945
Host Species :
Mouse
Product Name :
Myc Tag Antibody (aa410-420) LS-C72945
Specificity :
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa410-420
PresentationDesc :
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%); Human, Chimpanzee, Gorilla, Rat, Amoeba (91%); Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).
PurificationDesc :
Protein G purified
IsotypeName :
IgG1
Gene :
Myc Tag
Usage :
IP, WB
ShortWebDescription :
Myc Tag antibody LS-C72945 is an unconjugated mouse monoclonal antibody to Myc Tag (aa410-420). Validated for IP and WB.
UsageText :
Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 ug/ml detects Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag. Lysates from NIH/3T3 cells transfected with either empty vector, PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLLRKRGST, or PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLNGST were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Myc Tag (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Myc-tagged PP2A A subunit (~63kD). Longer exposure demonstrating weak detection by anti-Myc Tag of the Myc-tagged protein containing the epitope tag sequence MEQKLISEEDLNGST. Immunoprecipitation: Reported that this antibody immunoprecipitates Myc-tagged protein from transfected cells.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.