This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Acetyl-Lysine Antibody LS-C71875
catalog :
LS-C71875
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, immunoprecipitation
product information
AntibodyID :
72501
AntibodyName :
LS-C71875
Host Species :
Rabbit
Product Name :
Acetyl-Lysine Antibody LS-C71875
Specificity :
Recognizes acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.05% Sodium Azide, 30% Glycerol
ImmunogenType :
Hapten
ImmunogenDesc :
A mixture of chemically acetylated antigens.
PurificationDesc :
Antiserum
IsotypeName :
IgG
Gene :
Acetyl-Lysine
Usage :
IP (1:1000 - 1:2000), WB
ShortWebDescription :
Acetyl-Lysine antibody LS-C71875 is an unconjugated rabbit polyclonal antibody to Acetyl-Lysine. Validated for IP and WB.
UsageText :
Suitable for use in Western Blot and Immunoprecipitation. Western Blot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C). Immunoprecipitation: 5 ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.