product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
Firefly Luciferase Antibody LS-C71819
catalog :
LS-C71819
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
Photinus, Pyralis
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; Pyralis
  • western blot; Photinus
Chen J, Huang C, Zhang B, Yin S, Liang J, Xu C, et al. Mutations of RagA GTPase in mTORC1 Pathway Are Associated with Autosomal Dominant Cataracts. PLoS Genet. 2016;12:e1006090 pubmed publisher
product information
AntibodyID :
72445
AntibodyName :
LS-C71819
TargetSpecies :
Photinus pyralis
Host Species :
Mouse
Product Name :
Firefly Luciferase Antibody LS-C71819
Specificity :
This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Luciferase protein from Photinus pyralis.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
Firefly Luciferase
Reactivity :
Photinus pyralis
Usage :
IF (10 - 30 µg/ml), IHC, IHC-Fr, WB (1 - 3 µg/ml)
ShortWebDescription :
Firefly Luciferase antibody LS-C71819 is an unconjugated mouse monoclonal antibody to photinus pyralis Firefly Luciferase. Validated for IF, IHC and WB. Cited in 1 publication.
UsageText :
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 ug/ml.
Synonyms :
, Firefly Luciferase, PPLUC
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.