This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IL15RA Antibody (Extracellular Domain) LS-C70951
catalog :
LS-C70951
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, neutralization, flow cytometry
product information
AntibodyID :
71577
AntibodyName :
LS-C70951
TargetSpecies :
Mouse
Host Species :
Goat
Product Name :
IL15RA Antibody (Extracellular Domain) LS-C70951
Specificity :
Recognizes mouse IL-15Ra.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Extracellular Domain
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
NS0-derived rmIL-15Ra extracellular domain.
PurificationDesc :
Immunoaffinity purified
ResuspensionLiquidDesc :
Phosphate Buffered Saline (PBS)
IsotypeName :
IgG
Gene :
IL15RA
StandardGeneSymbol :
IL15RA
gene family :
Interleukin
Reactivity :
Mouse
Usage :
ELISA, Flo, Func, Neut, WB
ShortWebDescription :
IL15RA antibody LS-C70951 is an unconjugated goat polyclonal antibody to mouse IL15RA (Extracellular Domain). Validated for ELISA, Flow, Func, Neut and WB.
UsageText :
Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 ug/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5% cross-reactivity with rhIL-15 R and less than 1% cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 ug/mL/1E6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.
Synonyms :
IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.