This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IL9R / CD129 Antibody (Extracellular Domain) LS-C70936
catalog :
LS-C70936
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
neutralization, flow cytometry
product information
AntibodyID :
71562
AntibodyName :
LS-C70936
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IL9R / CD129 Antibody (Extracellular Domain) LS-C70936
Specificity :
Recognizes human IL-9R. In direct ELISAs, this antibody does not cross-react with rhIL-4 R, rhIL-5 R, rhIL-5 R, rhIL-13 R 1, rhIL-13 R 2, or rmIL-9 R.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
Extracellular Domain
PresentationDesc :
Lyophilized from PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Sf21-derived rhIL-9 R extracellular domain.
PurificationDesc :
Protein G purified
ResuspensionLiquidDesc :
Phosphate Buffered Saline (PBS)
IsotypeName :
IgG1
Gene :
IL9R / CD129
StandardGeneSymbol :
IL9R
gene family :
Interleukin
Reactivity :
Human
Usage :
ELISA, Flo, Func, Neut
ShortWebDescription :
CD129 antibody LS-C70936 is an unconjugated mouse monoclonal antibody to human CD129 (IL9R) (Extracellular Domain). Validated for ELISA, Flow, Func and Neut.
UsageText :
Neutralization of bioactivity, Flow Cytometry, Direct ELISA. Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4 ug/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10^5 cells/mL) in the presence of 2 ng/mL of rhIL-9. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well.
Synonyms :
IL9R, IL-9 receptor, IL-9R, Interleukin 9 receptor, Interleukin-9 receptor, CD129, CD129 antigen
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.