antibody

IL6 / Interleukin 6 Antibody LS-C70904

LS-C70904

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

LS-C70904

Human

Rabbit

IL6 / Interleukin 6 Antibody LS-C70904

The antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i. e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

Polyclonal

Unconjugated

PBS, pH 7.2.

Recombinant protein

Recombinant human IL-6 produced in E. coli.

Antiserum

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

IgG

IL6 / Interleukin 6

IL6

Hormone

Mouse, Rat, Human

ELISA (1:1000 - 1:5000), IHC, IHC-Fr, IHC-P, IP (1:400 - 1:800), Neut (1:400), WB

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, and Neutralization. ELISA: 1:1000-1:5000. Western Blot: Detects IL-6 in supernatants and lysates of 2x1E6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PMBC are stimulated for 24 hours with 1% human serum plus 10 ng/ml E. coli LPS. Multiple bands bands will appear due to the variable amounts of glycosylation on the molecule (21-21kD). Immunoprecipitation: 1:400-1:800; Precleaning with a non-specific rabbit IgG reduces background. Immunohistochemistry (Paraffin) neutral buffered formalin fixed: 1:400-1:800. Immunohistochemistry (Frozen) methanol or acetone fixed: 1:400-1:800. Neutralization: 1:400. Incubate with sample for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6