This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FZD4 / Frizzled 4 Antibody (Biotin) LS-C6905
catalog :
LS-C6905
clonality :
monoclonal
host :
rat
conjugate :
biotin
reactivity :
human, mouse
application :
western blot, immunohistochemistry, flow cytometry
product information
AntibodyID :
7646
AntibodyName :
LS-C6905
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
FZD4 / Frizzled 4 Antibody (Biotin) LS-C6905
Specificity :
Recognizes human and mouse Frizzled-4. Does not cross-react with rmFrizzled-1, -2, -3, -6, -7 or -8 in Western Blot. Detects Frizzled-4 by Flow Cytometry and Immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.
ClonalityDesc :
Monoclonal
AntibodyModification :
Biotin
PresentationDesc :
PBS, pH 7.2, 0.5% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
NS0-derived rmFrizzled-4 cysteine-rich domain.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with 1 ml sterile PBS.
IsotypeName :
IgG2a
Gene :
FZD4 / Frizzled 4
StandardGeneSymbol :
FZD4
gene family :
GPCR
Subfamily :
Frizzled
Reactivity :
Mouse, Human
Usage :
Flo, IHC (10 µg/ml), WB (1 - 2 µg/ml)
ShortWebDescription :
Frizzled 4 antibody LS-C6905 is a biotin-conjugated rat monoclonal antibody to Frizzled 4 (FZD4) from mouse. It is reactive with human and mouse. Validated for Flow, IHC and WB.
UsageText :
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Flow Cytometry: 0.1 mg/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of biotinylated monoclonal antibodies may be visualized by adding streptavidin conjugated to a fluorochrome. Immunohistochemistry: 10 ug/ml with the appropriate secondary reagents to detect Frizzled-4 in fixed human HEK293 cells. Western Blot: 1-2 ug/ml with the appropriate secondary reagents to detect human/mouse Frizzled-4. The detection limit for rmFrizzled-4 is ~5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.