This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FZD1 / Frizzled 1 Antibody (Extracellular Domain, Biotin) LS-C6896
catalog :
LS-C6896
clonality :
monoclonal
host :
rat
conjugate :
biotin
reactivity :
human, mouse
application :
western blot, immunocytochemistry, flow cytometry
product information
AntibodyID :
7637
AntibodyName :
LS-C6896
TargetSpecies :
Mouse
Host Species :
Rat
Product Name :
FZD1 / Frizzled 1 Antibody (Extracellular Domain, Biotin) LS-C6896
Specificity :
Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.
ClonalityDesc :
Monoclonal
AntibodyModification :
Biotin
AntigenModification :
Extracellular Domain
PresentationDesc :
PBS, pH 7.2, BSA
ImmunogenDesc :
NS0-derived rmFrizzled-1 extracellular domain.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG2a
Gene :
FZD1 / Frizzled 1
StandardGeneSymbol :
FZD1
gene family :
GPCR
Subfamily :
Frizzled
Reactivity :
Mouse, Human
Usage :
Flo, ICC, WB
ShortWebDescription :
Frizzled 1 antibody LS-C6896 is a biotin-conjugated rat monoclonal antibody to Frizzled 1 (FZD1) (Extracellular Domain) from mouse. It is reactive with human and mouse. Validated for Flow, ICC and WB.
UsageText :
Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/ml and add 10 ul of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ml of a 25 mg/ml stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 ug/ml in 4%. paraformaldehyde-fixed cells. Western Blot: Use at 1-2 ug/ml with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.