antibody

GZMB / Granzyme B Antibody (clone 351927) LS-C67063

LS-C67063

monoclonal

mouse

nonconjugated

351927

The same clone is also sold as:

• Invitrogen: MA5-23639, MA5-23688, MA5-23590
• R&D Systems: IC2906C, IC2906U-100UG, IC2906T-100UG, IC2906R-100UG, IC2906G, IC2906N-100UG, MAB2906, IC2906S-100UG, IC2906P, IC2906V-100UG

western blot, ELISA, immunohistochemistry, flow cytometry

LS-C67063

Human

Mouse

GZMB / Granzyme B Antibody (clone 351927) LS-C67063

Recognizes human Granzyme B. Shows no cross-reactivity with rhGranzyme A, -H, rmGranzyme B, -C, -D, or -G.

Monoclonal

351927

Unconjugated

Lyophilized from PBS, 5% Trehalose

Recombinant protein

NS0-derived rhGranzyme B.

Protein G purified

Lyophilized and resuspended forms: store at -20°C.

Reconstitute in 200 ul sterile PBS

IgG2a

GZMB / Granzyme B

GZMB

Protease

Serine S1

Human

ELISA, Flo, IHC, WB

Granzyme B antibody LS-C67063 is an unconjugated mouse monoclonal antibody to human Granzyme B (GZMB). Validated for ELISA, Flow, IHC and WB.

Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human Granzyme B. Using a colorimetric detection system, the detection limit for rhGranzyme B is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was tested for flow cytometry using NK-92 cells. For intracellular staining to detect human Granzyme B, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. Following a 30 minute incubation, cells should be washed with 0.1% saponin prior to addition of a secondary developing reagent. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunohistochemistry: This antibody was used at a concentration of 8-25 ug/mL with appropriate secondary reagents to detect human Granzyme B in paraformaldehyde-fixed human PBMC. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Granzyme B. The detection limit for rhGranzyme B is approximately 5 ng/well.

GZMB, CCPI, CGL-1, Cathepsin G-like 1, CSP-B, CTLA-1, CTLA1, CGL1, CSPB, Fragmentin 2, GRB, HLP, Human lymphocyte protein, Fragmentin-2, SECT, Lymphocyte protease, T-cell serine protease 1-3E, C11, CTSGL1, Cytotoxic serine protease B, Granzyme B, Granzyme-2

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