product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
NRSF / REST Antibody LS-C668231
catalog :
LS-C668231
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Pearsall S, Humphrey S, Revill M, Morgan D, Frese K, Galvin M, et al. The Rare YAP1 Subtype of SCLC Revisited in a Biobank of 39 Circulating Tumor Cell Patient Derived Explant Models: A Brief Report. J Thorac Oncol. 2020;15:1836-1843 pubmed publisher
product information
AntibodyID :
682681
AntibodyName :
LS-C668231
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
NRSF / REST Antibody LS-C668231
Specificity :
Recognizes endogenous levels of REST protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenDesc :
Recombinant full length protein of human REST
PurificationDesc :
Immunoaffinity chromatography
RecommendedStorageDesc :
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Gene :
NRSF / REST
StandardGeneSymbol :
REST
Reactivity :
Human
Usage :
ICC, IF (1:50 - 1:200), WB (1:500 - 1:2000)
ShortWebDescription :
REST antibody LS-C668231 is an unconjugated rabbit polyclonal antibody to human REST (NRSF). Validated for ICC, IF and WB. Cited in 1 publication.
UsageText :
Western blot analysis of REST expression in MCF7 (A), HL60 (B) whole cell lysates. Immunofluorescent analysis of REST staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Synonyms :
REST, X2 box repressor, XBR, NRSF
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.