This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FOXH1 Antibody (aa164-272) LS-C66336
catalog :
LS-C66336
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry
product information
AntibodyID :
66962
AntibodyName :
LS-C66336
TargetSpecies :
Human
Host Species :
Sheep
Product Name :
FOXH1 Antibody (aa164-272) LS-C66336
Specificity :
Recognizes human FoxH1. Species sequence homology: mouse 55%. less than 1% cross-reactivity with rhFoxB2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa164-272
PresentationDesc :
Lyophilized PBS, 5% Trehalose
ImmunogenType :
Recombinant protein
ImmunogenDesc :
E. coli-derived recombinant human rhFoxH1 (aa 164 - 272).
PurificationDesc :
Affinity purified
ResuspensionLiquidDesc :
Phosphate Buffered Saline (PBS)
IsotypeName :
IgG
Gene :
FOXH1
StandardGeneSymbol :
FOXH1
gene family :
Transcription factor
Reactivity :
Human
Usage :
ELISA (0.5 - 1 µg/ml), ICC (10 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
FOXH1 antibody LS-C66336 is an unconjugated sheep polyclonal antibody to human FOXH1 (aa164-272). Validated for ELISA, ICC and WB.
UsageText :
Suitable for use in Western blot, Immunocytochemistry and Direct ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~0.2 ng/well. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1. (rhFoxH1; aa 164-272; Accession # O75593).
Synonyms :
FOXH1, Fast-2, FAST2, FAST-1, FAST1, Forkhead box H1, Forkhead box protein H1, HFAST-1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.