product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
EGFR Antibody (Extracellular Domain, clone 225) LS-C6560
catalog :
LS-C6560
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
225
reactivity :
human
application :
immunoprecipitation, flow cytometry
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 10 ug/ml; loading ...; fig 4a
Day K, Lorenzatti Hiles G, Kozminsky M, Dawsey S, Paul A, Broses L, et al. HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone. Cancer Res. 2017;77:74-85 pubmed publisher
product information
AntibodyID :
7301
AntibodyName :
LS-C6560
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
EGFR Antibody (Extracellular Domain, clone 225) LS-C6560
Specificity :
Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. The epitope maps to the extracellular domain of EGFR. Species cross-reactivity: chimpanzee and monkey. Does not react with mouse or rat.
ClonalityDesc :
Monoclonal
CloneName :
225
AntibodyModification :
Unconjugated
AntigenModification :
Extracellular Domain
PresentationDesc :
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
ImmunogenType :
Cells
ImmunogenDesc :
Purified EGFR from A431 cells.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
EGFR
StandardGeneSymbol :
EGFR
gene family :
Protein Kinase
Subfamily :
EGF Receptor
Reactivity :
Human, Monkey
Usage :
IP
ShortWebDescription :
EGFR antibody LS-C6560 is an unconjugated mouse monoclonal antibody to EGFR (Extracellular Domain) from human. It is reactive with human and monkey. Validated for IP.
UsageText :
Suitable for use in Immunoprecipitation, Blocking EGT/TGFa-Induced Activation of EGFR and also Inhibits binding of E3375-03. Blocking EGF/TGFa-induced activation of EGFR: Use 0.5 ug/ml, cells induced by 30 ng/ml EGF. Immunoprecipitation (native only): 2 ug/mg protein lysate. Use Protein G. Positive control: A431 cells.
Synonyms :
EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.