This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Cytokeratin 5+8 Antibody (aa353-367) LS-C64333
catalog :
LS-C64333
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
64959
AntibodyName :
LS-C64333
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Cytokeratin 5+8 Antibody (aa353-367) LS-C64333
Specificity :
Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa353-367
PresentationDesc :
Lyophilized from PBS, pH 7.4, 0.09% Sodium Azide, 0.5% BSA
ImmunogenType :
Purified protein
ImmunogenDesc :
Human cytokeratin 8, purified from SDS PAGE.
PurificationDesc :
Protein A purified
ResuspensionLiquidDesc :
Sterile 40-50% glycerol.
IsotypeName :
IgG1
Gene :
Cytokeratin 5+8
Reactivity :
Human
Usage :
IHC, IHC-Fr, IHC-P, WB
ShortWebDescription :
Cytokeratin 5+8 antibody LS-C64333 is an unconjugated mouse monoclonal antibody to human Cytokeratin 5+8 (aa353-367). Validated for IHC and WB.
UsageText :
Suitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material) and Western Blot (Western). Immunohistochemistry: 1:10; Incubation Time 1h at RT, extended with paraffin sections (overnight at 2-8?C). Pretreatment with paraffin embedded sections, protease pretreatment is required prior to antibody application.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.