product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
CXCR3 Antibody (clone 2AR1) LS-C6283
catalog :
LS-C6283
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2AR1
reactivity :
human
application :
immunohistochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human
  • neutralization; human
Mikucki M, Fisher D, Matsuzaki J, Skitzki J, Gaulin N, Muhitch J, et al. Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints. Nat Commun. 2015;6:7458 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:20000
Denkert C, Loibl S, Noske A, Roller M, Müller B, Komor M, et al. Tumor-associated lymphocytes as an independent predictor of response to neoadjuvant chemotherapy in breast cancer. J Clin Oncol. 2010;28:105-13 pubmed publisher
product information
AntibodyID :
7024
AntibodyName :
LS-C6283
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CXCR3 Antibody (clone 2AR1) LS-C6283
Specificity :
Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not cross-react with CXCR1, CXCR2, CXCR4 or transfectants.
ClonalityDesc :
Monoclonal
CloneName :
2AR1
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 6.8, 5% Trehalose
ImmunogenType :
Cells
ImmunogenDesc :
CXCR3 transfected NS0 cells.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in 200 ul sterile PBS
IsotypeName :
IgG1
Gene :
CXCR3
StandardGeneSymbol :
CXCR3
gene family :
GPCR
Subfamily :
Chemokine
Reactivity :
Human
Usage :
Flo (5 - 10 µg/ml), Func, IHC (10 µg/ml), Neut
ShortWebDescription :
CXCR3 antibody LS-C6283 is an unconjugated mouse monoclonal antibody to human CXCR3. Validated for Flow, Func, IHC and Neut. Cited in 2 publications.
UsageText :
Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 ug/ml in the presence of 7 ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 ug/ml; Add 10 ul to 1-2.5x10^5 cells in total reaction volume 200ul. Visualize binding by adding 10 ul of 25 ug/ml IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 ug/ml detected CXCR3 in human tonsil. Cells were fixed in PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Cells were washed with PBS, BSA.
Synonyms :
CXCR3, C-x-c chemokine receptor 3, CD183 antigen, CKR-L2, CXCR-3, CMKAR3, CD182, CD183, Cxcr3a, GPR9, IP-10 receptor, IP10-R, Mig receptor, MigR, Mig-R, Chemokine (C-X-C) receptor 3, CXC-R3, Cxcr3b, G protein-coupled receptor 9, IP10 receptor
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.